|
|
Development of methods for genetic analysis of plant foods
Fialová, Lenka ; Brázda, Václav (referee) ; Doškař, Jiří (referee) ; Márová, Ivana (advisor)
Multiplex real-time PCR-HRM is an approach which has gained some attention in recent years. It has already found applications in clinical diagnostics and food authenticity and safety control. Compared to its corresponding singleplex PCR assays, an optimized multiplex PCR assay provides the same information in a fraction of time. First part of this work dealt with isolation of DNA from both fresh fruits and processed commercial products. Six different DNA isolation protocols were tested with fresh fruits – three silica column-based kits, two magnetic carrier-based kits and one conventional protocol. One method was chosen as the most suitable and was applied to DNA isolation from commercial products. These experiments also involved optimisation of the chosen method. The second part of this work was focused on the development of a triplex real-time PCR assay for simultaneous detection of blueberry, strawberry and raspberry, and its application on DNA isolated from commercial products. During DNA isolation, calcium chloride was shown to be a promising agent for removal of pectin from samples. In several samples, presence of raspberry DNA was confirmed by singleplex PCR. We found out that for accurate results of food analysis by this assay, further optimization of its parameters would be needed.
|
|
|
Technologické parametry a konzervárenské zpracování hrušek asijských odrůd
Jarolík, Ivan
Theoretical part of thesis contains information about importance of sensory analysis for quality evaluation of food. Further described are tools of sensory perception, which are used for sensory analysis. Experimental part consists of laboratory analysis of six varieties of pears in fresh form (Asian varieties Man san gill, Kumt ghant chu, Shinseiki, Chojuro and Pung su, and European variety Conference). These pears were then processed into juice, compote and puree. These products were evaluated in sensory analysis. It was found that the drought of summer 2015 had significant effect on size and weight of pears. Results of sensory analysis tell us that variety Man san gill is most suitable for production of the juice, Shinseiki for production of the compote and Conference for production of the puree. Asian pears are not suitable for the puree production.
|
|
|
Srovnání kvality plodů asijských a evropských hrušní
Jarolík, Ivan
Theoretical part of this bachelor's thesis contains brief history of pear cultivation from ancient Greece to expansion of pears in the USA. In practical part were four varieties of Asian pears (Mansan, Hosui, Pung su, Chojuro) and one variety of European pear (Dita) sensory evaluated as fresh and as processed into stew and purée. Asian pears were stored 40 days in temperature of 18°C. It was found, that the most suitable variety to be consumed as fresh is Mansan and most suitable variety to be processed into stew is Pung su. Furthermore, the evaluation results, that Asian pears are not suitable for processing into purée and it's preferable to use European varieties.
|
guest ::
